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Bio-Rad 0.4-cm gap width electroporation cuvette
NS2/C113 mutation has no effect on NS2 YFP -E2 interaction. The interaction of NS2 YFP with E2 and NS3 was determined by NS2 YFP pull-down from cell lysates prepared at day 3 post <t>electroporation</t> of 2 YFP E3 RNA. The data presented are representative of the results of three independent experiments.
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Bio-Rad electroporation cuvettes (0.4 cm gap, bio‐rad, hercules, ca, usa)
NS2/C113 mutation has no effect on NS2 YFP -E2 interaction. The interaction of NS2 YFP with E2 and NS3 was determined by NS2 YFP pull-down from cell lysates prepared at day 3 post <t>electroporation</t> of 2 YFP E3 RNA. The data presented are representative of the results of three independent experiments.
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NS2/C113 mutation has no effect on NS2 YFP -E2 interaction. The interaction of NS2 YFP with E2 and NS3 was determined by NS2 YFP pull-down from cell lysates prepared at day 3 post <t>electroporation</t> of 2 YFP E3 RNA. The data presented are representative of the results of three independent experiments.
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Fisher Scientific electroporation cuvette 0.4-cm gap
NS2/C113 mutation has no effect on NS2 YFP -E2 interaction. The interaction of NS2 YFP with E2 and NS3 was determined by NS2 YFP pull-down from cell lysates prepared at day 3 post <t>electroporation</t> of 2 YFP E3 RNA. The data presented are representative of the results of three independent experiments.
Electroporation Cuvette 0.4 Cm Gap, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NS2/C113 mutation has no effect on NS2 YFP -E2 interaction. The interaction of NS2 YFP with E2 and NS3 was determined by NS2 YFP pull-down from cell lysates prepared at day 3 post electroporation of 2 YFP E3 RNA. The data presented are representative of the results of three independent experiments.

Journal: Journal of Virology

Article Title: Palmitoylation of Hepatitis C Virus NS2 Regulates Its Subcellular Localization and NS2-NS3 Autocleavage

doi: 10.1128/JVI.00906-19

Figure Lengend Snippet: NS2/C113 mutation has no effect on NS2 YFP -E2 interaction. The interaction of NS2 YFP with E2 and NS3 was determined by NS2 YFP pull-down from cell lysates prepared at day 3 post electroporation of 2 YFP E3 RNA. The data presented are representative of the results of three independent experiments.

Article Snippet: For electroporation, 10 μg of RNA was mixed with 5 × 10 6 FT3-7 cells in a 0.4-cm gap width electroporation cuvette (Bio-Rad, Hercules, CA) and pulsed once at 270 V and 950 μF using a GenePulser system (Bio-Rad, Hercules, CA).

Techniques: Mutagenesis, Electroporation